Correlative imaging of collagen fibers and fibroblasts using CLEM optimized for picrosirius red staining and FIB/SEM tomography
نویسندگان
چکیده
منابع مشابه
Histochemical analysis of polarizing colors of collagen using Picrosirius Red staining in oral submucous fibrosis.
BACKGROUND Oral Submucous fibrosis (OSMF) is a common potentially malignant disease, which is characterized by generalized fibrosis of the oral soft tissues. It is due to disproportion in the collagen deposition and degradation. The excess deposition of collagen results in juxta epithelial fibro elastic changes and epithelial atrophy. Neoplastic changes in the epithelium usually precede the cha...
متن کاملCollagen and Picrosirius Red Staining: a Polarized Light Assessment of Fibrillar Hue and Spatial Distribution
Collagen plays a vital role in maintaining structural integrity and in determining tissue function. Therefore, methods to detect, quantify, and analyze collagen are valuable. Nevertheless, stains historically employed to detect collagen have disadvantages, principally a poor specificity for thin fibers. Conversely, picrosirius red, which has the capability to detect thin fibers, although freque...
متن کاملdevelopment and implementation of an optimized control strategy for induction machine in an electric vehicle
in the area of automotive engineering there is a tendency to more electrification of power train. in this work control of an induction machine for the application of electric vehicle is investigated. through the changing operating point of the machine, adapting the rotor magnetization current seems to be useful to increase the machines efficiency. in the literature there are many approaches wh...
15 صفحه اولPicrosirius red staining of cardiac muscle following phosphomolybdic acid treatment.
When the picrosirius red technique was applied to cardiac muscle sections, intense yellow myocyte staining sometimes obscured thin collagenous septa. The picrosirius red technique was modified to include treatment of the sections in 0.2% (w/v) aqueous phosphomolybdic acid prior to staining. With 1-5 min treatment, cytoplasmic staining was eradicated; diminution of collagen staining occurred onl...
متن کاملCalcium hydroxylapatite treatment of human skin: evidence of collagen turnover through picrosirius red staining and circularly polarized microscopy
Background Calcium hydroxylapatite (CaHA, Radiesse®) is a biocompatible, injectable filler for facial soft-tissue augmentation that provides volume to tissues, followed by a process of neocollagenesis for improved skin quality. Objective To examine the effects of CaHA treatment on the molecular organization of collagen using a combination of picrosirius red staining and circularly polarized l...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Microscopy
سال: 2020
ISSN: 2050-5701
DOI: 10.1093/jmicro/dfaa024